Poster: Introducing the hermes package for analyzing and reporting RNA-seq data

Introducing the hermes package for analyzing and reporting RNA-seq data

Daniel Sabanes Bove

Roche

Abstract

The hermes package published on Bioconductor provides classes and functions for quality control, filtering, normalization and differential expression analysis of pre-processed RNA-seq data. Data can be imported from SummarizedExperiment as well as matrix objects and can be annotated from BioMart. Filtering for genes without too low expression or containing required annotations, as well as filtering for samples with sufficient correlation to other samples or total number of reads is supported. The standard normalization methods including cpm, rpkm and tpm can be used, and DESeq2 as well as voom differential expression analyses are available. We present the package, as well as a (still closed but soon open source) extension including Shiny modules based on the “teal” framework.